A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction
Ceramides are synthesized by six mammalian ceramide synthases (CerSs), each of which uses fatty acyl-CoAs of different chain lengths for N-acylation of the sphingoid long-chain base. We now describe a rapid and reliable CerS assay that uses a fluorescent N-[6-[(7-nitrobenzo-2-oxa-1,3-diazol-4-yl) (N...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2015-01-01
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| Serie: | Journal of Lipid Research |
| Soggetti: | |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S0022227520356522 |
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