Improved Method for the Production of M13 Phage and Single-Stranded DNA for DNA Sequencing
An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F′) cells with as few as 5 phage particles can yield 1012 phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth s...
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| Principais autores: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
1996-05-01
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| Serier: | BioTechniques |
| Online adgang: | https://www.future-science.com/doi/10.2144/96205st05 |
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