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Improved Method for the Production of M13 Phage and Single-Stranded DNA for DNA Sequencing

An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F′) cells with as few as 5 phage particles can yield 1012 phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth s...

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Bibliografski detalji
Glavni autori: Prasad Reddy, Keith McKenney
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis Group 1996-05-01
Serija:BioTechniques
Online pristup:https://www.future-science.com/doi/10.2144/96205st05
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