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Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.

BACKGROUND: Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different protein of interest. However, the relative expression levels may vary strongly between individual cells and cannot be controlled. Ideal...

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Principais autores: Joachim Goedhart, Laura van Weeren, Merel J W Adjobo-Hermans, Ies Elzenaar, Mark A Hink, Theodorus W J Gadella
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Acceso en liña:http://europepmc.org/articles/PMC3219669?pdf=render
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