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A FRET-based biosensor for measuring Gα13 activation in single cells.

Förster Resonance Energy Transfer (FRET) provides a way to directly observe the activation of heterotrimeric G-proteins by G-protein coupled receptors (GPCRs). To this end, FRET based biosensors are made, employing heterotrimeric G-protein subunits tagged with fluorescent proteins. These FRET based...

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Bibliografische gegevens
Hoofdauteurs: Marieke Mastop, Nathalie R Reinhard, Cristiane R Zuconelli, Fenna Terwey, Theodorus W J Gadella, Jakobus van Unen, Merel J W Adjobo-Hermans, Joachim Goedhart
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science (PLoS) 2018-01-01
Reeks:PLoS ONE
Online toegang:http://europepmc.org/articles/PMC5837189?pdf=render
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