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Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution

Summary: Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs in...

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Príomhchruthaitheoirí: Diane Bissen, Maximilian Ken Kracht, Franziska Foss, Amparo Acker-Palmer
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Elsevier 2022-09-01
Sraith:STAR Protocols
Ábhair:
Rochtain ar líne:http://www.sciencedirect.com/science/article/pii/S2666166722003872
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