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Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specim...

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Автори: Ya-Hui Lin, Li-Wen Wang, Yen-Hui Chen, Yi-Chieh Chan, Shang-Hsiu Hu, Sheng-Yan Wu, Chi-Shiun Chiang, Guan-Jie Huang, Shang-Da Yang, Shi-Wei Chu, Kuo-Chuan Wang, Chin-Hsien Lin, Pei-Hsin Huang, Hwai-Jong Cheng, Bi-Chang Chen, Li-An Chu
Формат: Artigo
Мова:Inglês
Опубліковано: eLife Sciences Publications Ltd 2024-05-01
Серія:eLife
Предмети:
tissue clearing
multiplexed staining
FFPE
archived tissues
whole mouse brain imaging
whole brain analysis
Онлайн доступ:https://elifesciences.org/articles/93212
Теги: Додати тег
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