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Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast

Abstract To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA reveals that most mRNA synthesis rates are around several transcripts per cell and cell cycle, and...

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Principais autores: Christian Miller, Björn Schwalb, Kerstin Maier, Daniel Schulz, Sebastian Dümcke, Benedikt Zacher, Andreas Mayer, Jasmin Sydow, Lisa Marcinowski, Lars Dölken, Dietmar E Martin, Achim Tresch, Patrick Cramer
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Nature 2011-01-01
coleção:Molecular Systems Biology
Assuntos:
gene expression
gene regulation
mRNA transcription
mRNA turnover
salt stress response
Acesso em linha:https://doi.org/10.1038/msb.2010.112
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