Selection of biophysically favorable antibody variants using a modified Flp-In CHO mammalian display platform

Mammalian display enables the selection of biophysically favorable antibodies from a large IgG antibody library displayed on the plasma membrane of mammalian cells. We constructed and validated a novel mammalian display platform utilizing the commercially available Flp-In CHO cell line as a starting...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Olli Huhtinen, Rune Salbo, Urpo Lamminmäki, Stuart Prince
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2023-05-01
Schriftenreihe:	Frontiers in Bioengineering and Biotechnology
Schlagworte:	antibody engineering mammalian display Bxb1 integrase recombinase mediated cassette exchange (RMCE) Flp-In CHO antibody developability
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1170081/full
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