Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals
In this paper, we describe a rapid and accurate real-time quantitative PCR-based system to determine transgene copy number in transgenic animals. We used the 2−ΔΔCt method to analyze different transgenic lines without the requirement of a control sample previously determined by Southern blot analysi...
Shranjeno v:
| Principais autores: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Taylor & Francis Group
2004-10-01
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| Serija: | BioTechniques |
| Online dostop: | https://www.future-science.com/doi/10.2144/04374ST06 |
| Oznake: |
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