QR koda

Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals

In this paper, we describe a rapid and accurate real-time quantitative PCR-based system to determine transgene copy number in transgenic animals. We used the 2−ΔΔCt method to analyze different transgenic lines without the requirement of a control sample previously determined by Southern blot analysi...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Principais autores: Maria Ballester, Anna Castelló, Elena Ibáñez, Armand Sánchez, Josep M. Folch
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis Group 2004-10-01
Serija:BioTechniques
Online dostop:https://www.future-science.com/doi/10.2144/04374ST06
Oznake: Označite
Brez oznak, prvi označite!