Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals
In this paper, we describe a rapid and accurate real-time quantitative PCR-based system to determine transgene copy number in transgenic animals. We used the 2−ΔΔCt method to analyze different transgenic lines without the requirement of a control sample previously determined by Southern blot analysi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis Group
2004-10-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04374ST06 |
| Tags: |
No Tags, Be the first to tag this record!
|