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Enhancing soluble expression and purification of a bispecific scFv antibody in Escherichia coli using chaperones

Background and purpose: The use of a bispecific antibody engineered as a tandem single-chain fragment variable antibody (scFv-BsAb) offers several advantages over full-length antibodies, including enhanced tissue penetration and the potential for production in bacterial cells. However, production of...

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Detalhes bibliográficos
Principais autores: Zahra Alidousti-Shahraki, Afrooz Amini-Dehkordi, Nafiseh Esmaeil, Vajihe Akbari
Formato: Artigo
Idioma:Inglês
Publicado em: Wolters Kluwer Medknow Publications 2026-05-01
Colecção:Research in Pharmaceutical Sciences
Assuntos:
Acesso em linha:https://journals.lww.com/10.4103/RPS.RPS_10_24
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