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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome

The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the transcriptomes of isolated human chondrocytes no longe...

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Principais autores: Ping Shen, Peihua Wu, Tazio Maleitzke, Marie-Jacqueline Reisener, Gitta A. Heinz, Frederik Heinrich, Pawel Durek, Clemens Gwinner, Tobias Winkler, Matthias Pumberger, Carsten Perka, Mir-Farzin Mashreghi, Max Löhning
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2022-11-01
Serier:Frontiers in Bioengineering and Biotechnology
Fag:
chondrocyte isolation
human articular cartilage
small chondrocytes
large chondrocytes
transcriptome preservation
single-cell RNA sequencing
Online adgang:https://www.frontiersin.org/articles/10.3389/fbioe.2022.1046127/full
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