Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome

The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the transcriptomes of isolated human chondrocytes no longe...

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Autors principals:	Ping Shen, Peihua Wu, Tazio Maleitzke, Marie-Jacqueline Reisener, Gitta A. Heinz, Frederik Heinrich, Pawel Durek, Clemens Gwinner, Tobias Winkler, Matthias Pumberger, Carsten Perka, Mir-Farzin Mashreghi, Max Löhning
Format:	Artigo
Idioma:	Inglês
Publicat:	Frontiers Media S.A. 2022-11-01
Col·lecció:	Frontiers in Bioengineering and Biotechnology
Matèries:	chondrocyte isolation human articular cartilage small chondrocytes large chondrocytes transcriptome preservation single-cell RNA sequencing
Accés en línia:	https://www.frontiersin.org/articles/10.3389/fbioe.2022.1046127/full
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