上调microRNA-205表达对HeLa细胞生物学特性的影响
【目的】 探讨上调microRNA-205(miR-205)表达对HeLa细胞生物学特性的影响65377;【方法】 实验分4组:miR-205上调组,阴性对照组,空白转染组和空白对照组65377;每种检测重复3次65377;利用脂质体siPORT NeoFX Transfection Agent,向miR-205上调组细胞转染Pre-miR-hsa-miR-205 miRNA Precursor,阴性对照组细胞转染Cy3 dye labeled PremiR Negative Control65377;荧光显微镜下评估转染情况,实时定量PCR方法检测miR-205表达,四甲基偶氮唑蓝(MTT)...
Wedi'i Gadw mewn:
| Fformat: | Artigo |
|---|---|
| Iaith: | Chinês |
| Cyhoeddwyd: |
Editorial Office of Journal of Sun Yat-sen University
2009-01-01
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| Cyfres: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Pynciau: | |
| Mynediad Ar-lein: | http://xuebaoyx.sysu.edu.cn/zh/article/43579233/ |
| Tagiau: |
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
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