A novel vector for positive selection of inserts harboring an open reading frame by translational coupling
We have developed a novel vector, pTCS, as a tool for efficient selection of open reading frame (ORF)-containing inserts. In pTCS clones containing an insert with an ORF, a downstream marker gene (immE3, conferring resistance to colicin) is activated via translational coupling with the insert, and t...
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| Principais autores: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
2007-12-01
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| Serier: | BioTechniques |
| Online adgang: | https://www.future-science.com/doi/10.2144/000112629 |
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