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Protocol to assess RNA-RNA interactions in situ using an RNA-proximity ligation assay

Summary: Here, we describe a protocol to assess RNA-RNA interactions in situ using an adapted proximity ligation assay (PLA). We detail steps to perform RNA-probe hybridization, in situ rolling circle amplification, and immunofluorescence confocal microscopy. With these tools, it is possible to dete...

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Principais autores: Megha G. Basavappa, Jorge Henao-Mejia, Sara Cherry
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2022-12-01
Serier:STAR Protocols
Fag:
Single Cell
Microscopy
Molecular Biology
In Situ Hybridization
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166722007729
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