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An optimized proximity ligation assay to detect telomere dysfunction induced foci in human and mouse cells

Summary: Telomere dysfunction-induced foci (TIF) can be measured by immunofluorescence, combined with telomere-fluorescent in situ hybridization. We modified this approach by combining the proximity ligation assay (PLA), which detects colocalization of two molecules in proximity through a signal amp...

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Bibliografische gegevens
Hoofdauteurs: Yajun Wang, Luigi Ferrucci, Michael M. Seidman, Yie Liu
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2022-09-01
Reeks:STAR Protocols
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Online toegang:http://www.sciencedirect.com/science/article/pii/S2666166722004907
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