A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be image...

Täydet tiedot

Bibliografiset tiedot

Päätekijät:	Sonal Nagarkar-Jaiswal, Pei-Tseng Lee, Megan E Campbell, Kuchuan Chen, Stephanie Anguiano-Zarate, Manuel Cantu Gutierrez, Theodore Busby, Wen-Wen Lin, Yuchun He, Karen L Schulze, Benjamin W Booth, Martha Evans-Holm, Koen JT Venken, Robert W Levis, Allan C Spradling, Roger A Hoskins, Hugo J Bellen
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	eLife Sciences Publications Ltd 2015-03-01
Sarja:	eLife
Aiheet:	RMCE deGradFP iGFPi bruchpilot discs large 1 dunce
Linkit:	https://elifesciences.org/articles/05338
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