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Use and Evaluation of a pES213-Derived Plasmid for the Constitutive Expression of gfp Protein in Pathogenic Vibrios: a Tagging Tool for In Vitro Studies

ABSTRACT Insertion of green fluorescent protein (GFP) into bacterial cells for constitutive expression is a powerful tool for the localization of species of interest within complex mixtures. Here, we demonstrate and evaluate the efficacy of the pES213-derived donor plasmid pVSV102 (gfp Knr) as a con...

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Autori principali: William A. Norfolk, Erin K. Lipp
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2023-02-01
Serie:Microbiology Spectrum
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Accesso online:https://journals.asm.org/doi/10.1128/spectrum.02490-22
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