Fluorescence-Based Protein Footprinting Using Histidine-Tagged Protein
We describe a procedure for protein footprinting to identify the region(s) of a protein that interacts with a ligand. The method utilized the affinity of a stretch of histidine residues cloned into the protein to metalchelated resin. After limited protease digestion, the histidine-tagged end fragmen...
Na minha lista:
| Principais autores: | , |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
1998-01-01
|
| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/98241st04 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|