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Attachment of Histidine Tags to Recombinant Tumor Necrosis Factor-Alpha Drastically Changes Its Properties

When studying two different histidine tags attached to the N-termini of the trimeric cytokine tumor necrosis factor alpha (TNF), the biological activity — measured as cytotoxicity on the L-929 cell line — of both tagged proteins was drastically reduced. The longer His10 tag reduced cytotoxicity to a...

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-д хадгалсан:
Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Irena Fonda, Maja Kenig, Vladka Gaberc-Porekar, Primo Pristovaek, Viktor Menart
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: Wiley 2002-01-01
Цуврал:The Scientific World Journal
Онлайн хандалт:http://dx.doi.org/10.1100/tsw.2002.215
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