PCR optimization for buccal swab-derived samples: overcoming sporadic inhibition with bovine serum albumin

Abstract Background Buccal swabs are widely used as a non-invasive method for genomic DNA collection in large-scale genotyping studies. However, the sporadic presence of PCR inhibitors within these samples may hinder PCR amplification and affect assay reliability. This study investigates the incorpo...

Täydet tiedot

Bibliografiset tiedot

Päätekijät:	Stefanie Tietze, Julia Phieler, Sabine Bergmann, Juliane Grimmer, Kathrin Putke, Bastian Joffroy, Yannic Utz, Katrin Hengst, Bianca Heyn, Diana Panhans, Alexander H. Schmidt, Anja Klussmeier, Vinzenz Lange
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	BMC 2025-11-01
Sarja:	BMC Genomics
Aiheet:	Buccal swab-derived DNA PCR PCR inhibitors BSA HLA genotyping
Linkit:	https://doi.org/10.1186/s12864-025-12350-x
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