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PCR optimization for buccal swab-derived samples: overcoming sporadic inhibition with bovine serum albumin

Abstract Background Buccal swabs are widely used as a non-invasive method for genomic DNA collection in large-scale genotyping studies. However, the sporadic presence of PCR inhibitors within these samples may hinder PCR amplification and affect assay reliability. This study investigates the incorpo...

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Hoofdauteurs: Stefanie Tietze, Julia Phieler, Sabine Bergmann, Juliane Grimmer, Kathrin Putke, Bastian Joffroy, Yannic Utz, Katrin Hengst, Bianca Heyn, Diana Panhans, Alexander H. Schmidt, Anja Klussmeier, Vinzenz Lange
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BMC 2025-11-01
Reeks:BMC Genomics
Onderwerpen:
Buccal swab-derived DNA
PCR
PCR inhibitors
BSA
HLA genotyping
Online toegang:https://doi.org/10.1186/s12864-025-12350-x
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