Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner

The impact of antibody glycoforms on FcγRIIa activation and immune responses is poorly understood. Yet, glycoform binding assessment remains one of the major analytical challenges requiring long enrichment or glycoengineering steps. Here, we developed and applied an affinity capillary electrophoresi...

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Autori principali:	Christoph Gstöttner, Alexander Knaupp, Gestur Vidarsson, Dietmar Reusch, Tilman Schlothauer, Manfred Wuhrer, Elena Domínguez-Vega
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Frontiers Media S.A. 2022-09-01
Serie:	Frontiers in Immunology
Soggetti:	affinity capillary electrophoresis (ACE) mass spectrometry FcγRIIa receptor monoclonal antibody glycosylation interaction
Accesso online:	https://www.frontiersin.org/articles/10.3389/fimmu.2022.980291/full
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