Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner

The impact of antibody glycoforms on FcγRIIa activation and immune responses is poorly understood. Yet, glycoform binding assessment remains one of the major analytical challenges requiring long enrichment or glycoengineering steps. Here, we developed and applied an affinity capillary electrophoresi...

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Hlavní autoři:	Christoph Gstöttner, Alexander Knaupp, Gestur Vidarsson, Dietmar Reusch, Tilman Schlothauer, Manfred Wuhrer, Elena Domínguez-Vega
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Frontiers Media S.A. 2022-09-01
Edice:	Frontiers in Immunology
Témata:	affinity capillary electrophoresis (ACE) mass spectrometry FcγRIIa receptor monoclonal antibody glycosylation interaction
On-line přístup:	https://www.frontiersin.org/articles/10.3389/fimmu.2022.980291/full
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