Development of two multiplex PCR assays for rapid detection of eleven Gram-negative bacteria in children with septicemia

Abstract Aim This study aimed to develop a multiplex PCR assay for simultaneous detection of major Gram-negative etiologies of septicemia and evaluate its performance. Methods Multiplex PCR (mPCR) assays were developed targeting 11 bacterial strains. Species-specific primers were confirmed using kno...

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Detalhes bibliográficos

Principais autores:	Gabriel Miringu, Abednego Musyoki, Betty Muriithi, Ernest Wandera, Dan Waithiru, Erick Odoyo, Hisashi Shoji, Nelson Menza, Yoshio Ichinose
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2024-06-01
coleção:	Tropical Medicine and Health
Assuntos:	Septicemia mPCR Gram-negative bacteria Diagnosis
Acesso em linha:	https://doi.org/10.1186/s41182-024-00606-3
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