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Development of two multiplex PCR assays for rapid detection of eleven Gram-negative bacteria in children with septicemia

Abstract Aim This study aimed to develop a multiplex PCR assay for simultaneous detection of major Gram-negative etiologies of septicemia and evaluate its performance. Methods Multiplex PCR (mPCR) assays were developed targeting 11 bacterial strains. Species-specific primers were confirmed using kno...

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Uloženo v:
Hlavní autoři: Gabriel Miringu, Abednego Musyoki, Betty Muriithi, Ernest Wandera, Dan Waithiru, Erick Odoyo, Hisashi Shoji, Nelson Menza, Yoshio Ichinose
Médium: Artigo
Jazyk:Inglês
Vydáno: BMC 2024-06-01
Edice:Tropical Medicine and Health
Témata:
Septicemia
mPCR
Gram-negative bacteria
Diagnosis
On-line přístup:https://doi.org/10.1186/s41182-024-00606-3
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