Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli
Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and overexpression of endogenous pathway genes, respect...
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| Автори: | , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Frontiers Media S.A.
2023-11-01
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| Серія: | Frontiers in Bioengineering and Biotechnology |
| Предмети: | |
| Онлайн доступ: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1296132/full |
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