Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli

Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and overexpression of endogenous pathway genes, respect...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Jinho Kim, Taek Soon Lee
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2023-11-01
Schriftenreihe:	Frontiers in Bioengineering and Biotechnology
Schlagworte:	CRISPR interference isoprenol multiplexed CRISPRi arrays mevalonate pathway IPP-bypass pathway fed-batch cultivation
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1296132/full
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