Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
Abstract The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR c...
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| Principais autores: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
SpringerOpen
2017-05-01
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| Serier: | AMB Express |
| Fag: | |
| Online adgang: | http://link.springer.com/article/10.1186/s13568-017-0386-1 |
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