Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

Abstract The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR c...

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Autors principals:	Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma
Format:	Artigo
Idioma:	Inglês
Publicat:	SpringerOpen 2017-05-01
Col·lecció:	AMB Express
Matèries:	PCR cloning Prokaryotic expression Eukaryotic expression Lysis gene E Positive selection
Accés en línia:	http://link.springer.com/article/10.1186/s13568-017-0386-1
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