De novo basecalling of RNA modifications at single molecule and nucleotide resolution

Abstract RNA modifications influence RNA function and fate, but detecting them in individual molecules remains challenging for most modifications. Here we present a novel methodology to generate training sets and build modification-aware basecalling models. Using this approach, we develop the m 6 AB...

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Κύριοι συγγραφείς:	Sonia Cruciani, Anna Delgado-Tejedor, Leszek P. Pryszcz, Rebeca Medina, Laia Llovera, Eva Maria Novoa
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	BMC 2025-02-01
Σειρά:	Genome Biology
Θέματα:	Nanopore sequencing Native RNA RNA modifications Machine learning N6-methyladenosine Basecalling
Διαθέσιμο Online:	https://doi.org/10.1186/s13059-025-03498-6
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