Synthetic oligonucleotides as quantitative PCR standards for quantifying microbial genes

Real-time quantitative PCR (qPCR) has been widely used to quantify gene copy numbers in microbial ecology. Despite its simplicity and straightforwardness, establishing qPCR assays is often impeded by the tedious process of producing qPCR standards by cloning the target DNA into plasmids. Here, we de...

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Auteurs principaux:	Xingguo Han, Karin Beck, Helmut Bürgmann, Beat Frey, Beat Stierli, Aline Frossard
Format:	Artigo
Langue:	Inglês
Publié:	Frontiers Media S.A. 2023-10-01
Collection:	Frontiers in Microbiology
Sujets:	synthetic DNA plasmid real-time quantitative PCR qPCR standards qPCR methodology microbial and functional gene abundances
Accès en ligne:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1279041/full
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