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Synthetic oligonucleotides as quantitative PCR standards for quantifying microbial genes

Real-time quantitative PCR (qPCR) has been widely used to quantify gene copy numbers in microbial ecology. Despite its simplicity and straightforwardness, establishing qPCR assays is often impeded by the tedious process of producing qPCR standards by cloning the target DNA into plasmids. Here, we de...

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Main Authors: Xingguo Han, Karin Beck, Helmut Bürgmann, Beat Frey, Beat Stierli, Aline Frossard
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2023-10-01
Series:Frontiers in Microbiology
Subjects:
synthetic DNA
plasmid
real-time quantitative PCR
qPCR standards
qPCR methodology
microbial and functional gene abundances
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1279041/full
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