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Protocol to analyze tRNA and rRNA processing using biotin-labeled probes

Summary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells. We describe steps for separating RNA by denaturing elect...

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Autores principales: Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2025-06-01
Colección:STAR Protocols
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Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2666166725002722
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