Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes

The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, has been used to generate gene knock-outs with variable sizes of small insertion/deletions with high efficiency. More precise genome editing, e...

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Hlavní autoři:	Bangmei Wang, Kunyu Li, Amy Wang, Michelle Reiser, Thom Saunders, Richard F Lockey, Jia-Wang Wang
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis Group 2015-10-01
Edice:	BioTechniques
Témata:	CRISPR cleavage double-strand break (DSB) homology-directed-repair (HDR) embryonic stem (ES) cells zygote
On-line přístup:	https://www.future-science.com/doi/10.2144/000114339
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