Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes. In this study, the Gibson assembly and Gateway cloning combined Pyramiding Stac...

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Principais autores:	Dongdong Zeng, Cuiyuan Jing, Lin Tang, Peng He, Jie Zhang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2023-11-01
coleção:	Frontiers in Bioengineering and Biotechnology
Assuntos:	multigene stacking Gibson assembly gateway cloning pyramiding stacking of multigenes genetic engineering synthetic biology
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1263715/full
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