Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes. In this study, the Gibson assembly and Gateway cloning combined Pyramiding Stac...

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Egile Nagusiak:	Dongdong Zeng, Cuiyuan Jing, Lin Tang, Peng He, Jie Zhang
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	Frontiers Media S.A. 2023-11-01
Saila:	Frontiers in Bioengineering and Biotechnology
Gaiak:	multigene stacking Gibson assembly gateway cloning pyramiding stacking of multigenes genetic engineering synthetic biology
Sarrera elektronikoa:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1263715/full
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