MegaGate: A toxin-less gateway molecular cloning tool
Summary: Gateway cloning employs the use of the ccdb toxin and has low colony numbers, making it difficult to apply at scale to clone libraries of cDNA vectors. In this protocol, we describe MegaGate, a toxin-less Gateway technology capable of robust cDNA library cloning that is efficient, cheap, an...
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| Главные авторы: | , , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2021-12-01
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| Серии: | STAR Protocols |
| Предметы: | |
| Online-ссылка: | http://www.sciencedirect.com/science/article/pii/S2666166721006134 |
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