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Protocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs

Summary: We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the comprehensiveness of characteriz...

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Principais autores: Edmund Battey, Matthieu Dos Santos, Dipsikha Biswas, Pascal Maire, Kei Sakamoto
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2024-12-01
Serier:STAR Protocols
Fag:
Cell Biology
Cell isolation
Cell separation/fractionation
Metabolism
Microscopy
Model Organisms
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724005896
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