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Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

Abstract Background The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and r...

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Main Authors: Anna Gąciarz, Narendar Kumar Khatri, M. Lourdes Velez-Suberbie, Mirva J. Saaranen, Yuko Uchida, Eli Keshavarz-Moore, Lloyd W. Ruddock
Format: Artigo
Language:Inglês
Published: BMC 2017-06-01
Series:Microbial Cell Factories
Subjects:
Disulfide bonds
Cytoplasm
Escherichia coli
Fermentation
Fed-batch
Interleukin 6
Online Access:http://link.springer.com/article/10.1186/s12934-017-0721-x
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