Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

Abstract Background The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and r...

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Bibliografski detalji

Glavni autori:	Anna Gąciarz, Narendar Kumar Khatri, M. Lourdes Velez-Suberbie, Mirva J. Saaranen, Yuko Uchida, Eli Keshavarz-Moore, Lloyd W. Ruddock
Format:	Artigo
Jezik:	Inglês
Izdano:	BMC 2017-06-01
Serija:	Microbial Cell Factories
Teme:	Disulfide bonds Cytoplasm Escherichia coli Fermentation Fed-batch Interleukin 6
Online pristup:	http://link.springer.com/article/10.1186/s12934-017-0721-x
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