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Emulsion PCR made easy

Emulsion PCR (ePCR) is an important technique that permits amplification of DNA molecules in physically separated picoliter-volume water-in-oil droplets, and thus avoids formation of unproductive chimeras and other artifacts between similar DNA sequences. However, the recovery of ePCR products invol...

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Principais autores: Vaishali Verma, Amita Gupta, Vijay K Chaudhary
פורמט: Artigo
שפה:Inglês
יצא לאור: Taylor & Francis Group 2020-07-01
סדרה:BioTechniques
נושאים:
aptamer
chimerization
emulsion PCR
extraction
next-generation sequencing
silica column purification
גישה מקוונת:https://www.future-science.com/doi/10.2144/btn-2019-0161
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