High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli

Recombinant expression systems have become powerful tools for understanding the structure and function of proteins, including the apolipoproteins that comprise human HDL. However, human apolipoprotein (apo)A-II has proven difficult to produce by recombinant techniques, likely contributing to our lac...

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Hlavní autoři:	Loren E. Smith, Jun Yang, Leah Goodman, Xinqi Huang, Rong Huang, James Dressman, Jamie Morris, R. A. Gangani D. Silva, W. Sean Davidson, Giorgio Cavigiolio
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2012-08-01
Edice:	Journal of Lipid Research
Témata:	intein fusion protein affinity tag bacteria inexpensive low temperature cleavage
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S0022227520418759
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