Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae

Abstract Background Proteases are important enzymes in food and pharmaceutical industries, but challenges persist in their recombinant production due to host cell proteome hydrolysis and fitness loss. The development of recombinant expression systems for directed evolution of proteolytic enzymes, an...

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Bibliografiske detaljer

Principais autores:	Tova Lindh, Mattias Collin, Rolf Lood, Magnus Carlquist
Format:	Artigo
Sprog:	Inglês
Udgivet:	BMC 2025-05-01
Serier:	Microbial Cell Factories
Fag:	Protease expression Yeast expression system Recombinant enzyme production Bacterial protease engineering Saccharomyces cerevisiae host CRISPR/Cas9 integration
Online adgang:	https://doi.org/10.1186/s12934-025-02732-x
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