Protocol for rapid 5-plex 3D imaging and single-cell analysis of immune responses in whole murine lymph nodes

Summary: Lymph nodes orchestrate adaptive immune responses, with germinal centers enabling affinity maturation and plasma cell formation. Here, we present a protocol for rapid, high-resolution, multicolor 3D imaging of whole immunized mouse lymph nodes. We describe steps for immunization, lymph node...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Victoire Boulat, Alessandro Ciccarelli, Miu Shing Hung, Lingling Zhang, Anita Grigoriadis, Dinis Pedro Calado
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2025-09-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Cell Biology Immunology Microscopy
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166725004654
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