Protocol for rapid 5-plex 3D imaging and single-cell analysis of immune responses in whole murine lymph nodes

Summary: Lymph nodes orchestrate adaptive immune responses, with germinal centers enabling affinity maturation and plasma cell formation. Here, we present a protocol for rapid, high-resolution, multicolor 3D imaging of whole immunized mouse lymph nodes. We describe steps for immunization, lymph node...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Victoire Boulat, Alessandro Ciccarelli, Miu Shing Hung, Lingling Zhang, Anita Grigoriadis, Dinis Pedro Calado
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2025-09-01
coleção:	STAR Protocols
Assuntos:	Cell Biology Immunology Microscopy
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166725004654
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!