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Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells

Summary: CRISPR-Cas9 is a powerful technology for accurate and optimizable genome editing. Here, we present a protocol for generating monoclonal knockout (KO) cell lines using CRISPR-Cas9, ribonucleoprotein complexes (RNPs), and lipofection in adherent HNSCC cells from start to finish. We describe s...

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Autori principali: Fabian Geyer, Maximilian Geyer, Sarah Klapproth, Klaus-Dietrich Wolff, Markus Nieberler
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2023-09-01
Serie:STAR Protocols
Soggetti:
Cell Biology
Single Cell
Cancer
Sequencing
Molecular Biology
CRISPR
Accesso online:http://www.sciencedirect.com/science/article/pii/S2666166723003337
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