Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655
Abstract Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E....
Kaydedildi:
| Asıl Yazarlar: | , , , , |
|---|---|
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
SpringerOpen
2020-09-01
|
| Seri Bilgileri: | AMB Express |
| Konular: | |
| Online Erişim: | http://link.springer.com/article/10.1186/s13568-020-01099-z |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|